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Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers Scientia Agricola
Sousa,Adna Cristina Barbosa de; Godoy,Rodolfo; Sforça,Danilo Augusto; Campos,Tatiana de; Zucchi,Maria Imaculada; Jank,Liana; Souza,Anete Pereira de.
The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legumes; Transferability; Microsatellite; Germplasm.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400007
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Mating system parameters in a high density population of andirobas in the Amazon forest PAB
Campos,Tatiana de; Cunha,Marcia Oliveira da; Sousa,Adna Cristina Barbosa de; Teixeira,Renata Beltrão; Raposo,Andrea; Sebbenn,Alexandre Magno; Wadt,Lucia Helena de Oliveira.
The objective of this work was to estimate the mating system parameters of a andiroba (Carapa guianensis) population using microsatellite markers and the mixed and correlated mating models. Twelve open‑pollinated progeny arrays of 15 individuals were sampled in an area with C. guianensis estimated density of 25.7 trees per hectare. Overall, the species has a mixed reproductive system, with a predominance of outcrossing. The multilocus outcrossing rate (t m = 0.862) was significantly lower than the unity, indicating that self‑pollination occurred. The rate of biparental inbreeding was substantial (t m ‑ t s = 0.134) and significantly different from zero. The correlation of selfing within progenies was high (r s = 0.635), indicating variation in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carapa guianensis; Allogamy; Conservation; Genetic breeding; Microsatellite; Population size.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000500006
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Potato cultivar identification using molecular markers PAB
Rosa,Patricia Marchezi; Campos,Tatiana de; Sousa,Adna Cristina Barbosa de; Sforça,Danilo Augusto; Torres,Gisele Abigail Montan; Souza,Anete Pereira de.
The objective of this work was to evaluate a set of microsatellite markers for varietal identification and characterization of the most widespread potato cultivars in Brazil. The DNA from 14 potato cultivars was genotyped using microsatellite markers and the alleles were scored in silver-stained polyacrylamide gel. Twenty-four microsatellite markers were evaluated, and only one locus was monomorphic. Based on band patterns, a set of two microsatellites that were able to identify and differentiate all examined cultivars was obtained.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum; AFLP; Genetic certification; Varietal protection; Variety identification; RAPD.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000100015
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